ISO 23906-1:2020 pdf download

ISO 23906-1:2020 pdf download.Cigarettes — Determination of benzo[a]pyrene in cigarette mainstream smoke with an intense smoking regime using GC/MS
1 Scope
This document specifies a method for the determination of benzo[a]pyrene (B[a]P) in the total particulate matter (TPM) of cigarette mainstream smoke using gas chromatography/mass spectrometry (GC-MS) with methanol as extraction solvent. This method is specified using ISO 20778 smoking parameters. NOTE An alternative method for the determination of B[a]P is specified in ISO 23906-2 with a different clean-up using cyclohexane solvent and a shorter total analytical time.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 3402, Tobacco and tobacco products — Atmosphere for conditioning and testing
ISO 3696, Water for analytical laboratory use — Specification and test methods
ISO 8243, Cigarettes — Sampling
ISO 20778, Cigarettes — Routine analytical cigarette smoking machine — Definitions and standard conditions with an intense smoking regime
ISO 20779, Cigarettes — Generation and collection of total particulate matter using a routine analytical smoking machine with an intense smoking regime
3 Terms and definitions
No terms and definitions are listed in this document.
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5 Apparatus
The usual laboratory apparatus and equipment and, in particular, the following.
5.1 Routine analytical cigarette-smoking machine, complying with the requirements of ISO 20778 and equipped for smoking in accordance with ISO 20779.
5.2 Gas chromatograph with a mass selective detector, equipped with its computerized control and data acquisition and processing system. This system shall be able to pilot the mass spectrometer in order to obtain chromatographic data under single ion monitoring (SIM) detection mode. The gas chromatograph shall be configured to perform splitless injections on a capillary column. It is recommended to equip the gas chromatograph with an autosampler for sample injection.
5.3 Fused silica capillary column, for example a (5 %-phenyl)-methylpolysiloxane stationary phase and a 30 m length, 0,25 mm internal diameter column with a 0,25 µm film thickness are suitable for this analysis. NOTE Other columns can be used, provided that appropriate peak separation is obtained.
5.4 Rotary evaporator or equivalent equipment.
5.5 Vacuum sample preparation unit or equivalent equipment.
5.6 Solid phase extraction cartridges, cyclohexyl bonded silica phase volume of 6 ml and packed with 1 g is suitable.
5.7 Gas tight syringes or positive displacement pipettes, of capacities 25 µl, 100 µl, 250 µl and 1 000 µl.
5.8 General laboratory equipment, for the preparation of samples, standards and reagents. All glassware shall be cleaned before use to avoid any contamination. Amber glassware may be required.