ISO 6579:2017 pdf download

ISO 6579:2017 pdf download.Microbiology of the food chain — Horizontal method for the detection, enumeration and serotyping of Salmonella
1 Scope
This document specifies a horizontal method for the detection of Salmonella. It is applicable to the following:
— products intended for human consumption and the feeding of animals;
— environmental samples in the area of food production and food handling;
— samples from the primary production stage such as animal faeces, dust, and swabs.
With this horizontal method, most of the Salmonella serovars are intended to be detected. For the detection of some specific serovars, additional culture steps may be needed. For Salmonella Typhi and Salmonella Paratyphi, the procedure is described in Annex D.
The selective enrichment medium modified semi-solid Rappaport-Vassiliadis (MSRV) agar is intended for the detection of motile Salmonella and is not appropriate for the detection of non-motile Salmonella strains.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 6887 (all parts), Microbiology of food and animal feed — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination
ISO 7218, Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations
ISO 11133:2014, Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
4.3 Enrichment in/on selective media
Rappaport-Vassiliadis medium with soya (RVS broth) or Modified Semi-solid Rappaport-Vassiliadis (MSRV) agar and Muller-Kauffmann tetrathionate-novobiocin broth (MKTTn broth) are inoculated with the culture obtained in 4.2. The RVS broth or the MSRV agar is incubated at 41,5 °C for 24 h and the MKTTn broth at 37 °C for 24 h. For some products, it may be necessary to incubate the selective enrichment medium/media for an additional 24 h. NOTE MSRV agar is intended for the detection of motile Salmonella strains and is not appropriate for the detection of non-motile Salmonella strains.
7 Sampling
Sampling is not part of the method specified in this document (see the specific International Standard dealing with the product concerned). If there is no specific International Standard, it is recommended that the parties concerned come to an agreement on this subject. A recommended sampling method is given in ISO/TS 17728 [26] for food and animal feed, in ISO 707 [27 ] for milk and milk products, in ISO 13307 [28] for sampling at the primary production stage, in ISO 17604 [29] for sampling of carcasses, and in ISO 18593 [25] for sampling of surfaces. It is important that the laboratory receives a sample which is representative and has not been damaged or changed during transport or storage.