ISO 21149:2017 pdf download

ISO 21149:2017 pdf download.Cosmetics — Microbiology — Enumeration and detection of aerobic mesophilic bacteria
1 Scope
This document gives general guidelines for enumeration and detection of aerobic mesophilic bacteria present in cosmetics
— by counting the colonies on agar medium after aerobic incubation, or
— by checking the absence of bacterial growth after enrichment.
Because of the large variety of cosmetic products within this field of application, this method may not be appropriate for some products in every detail (e.g. certain water immiscible products). Other methods (e.g. automated) may be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable. If needed, microorganisms enumerated or detected may be identified using suitable identification tests described in the standards given in the Bibliography. In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis to determine the types of cosmetic products to which this document is applicable. Products considered to present a low microbiological risk (see ISO 29621) include those with low water activity, hydro-alcoholic products, extreme pH values, etc.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 21148:2017, Cosmetics — Microbiology — General instructions for microbiological examination
EN 12353, Chemical disinfectants and antiseptics — Preservation of test organisms used for the determination of bactericidal (including Legionella), mycobactericidal, sporicidal, fungicidal and virucidal (including bacteriophages) activity
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— IEC Electropedia: available at http:// w w w.electropedia.org/
— ISO Online browsing platform: available at http:// w w w.iso.org/obp
3.1 aerobic mesophilic bacterium
mesophilic bacterium growing aerobically under the conditions specified in this document
Note 1 to entry: In the described conditions, other types of microorganisms (e.g. yeast, mould) can be detected.
4.4 Detection of bacteria by enrichment
Detection of bacteria by enrichment consists of the following steps.
a) Incubation at 32,5 °C ± 2,5 °C for at least 20 h of a defined quantity of the initial suspension in a non-selective liquid medium containing suitable neutralizers and/or dispersing agents.
b) Transfer of a defined quantity of the previous suspension on non-selective solid agar medium.
c) Aerobic incubation at 32,5 °C ± 2,5 °C for 48 h to 72 h.
d) Detection of growth and expression of results as “presence/absence” of aerobic mesophilic bacteria per sample S of product.