BS ISO 18862:2016 pdf download

BS ISO 18862:2016 pdf download.Coffee and coffee products — Determination of acrylamide — Methods using HPLC-MS/MS and GC-MS after derivatization
1 Scope
This document specifies methods for the determination of acrylamide in coffee and coffee products by extraction with water, clean-up by solid-phase extraction and determination by HPLC-MS/MS and GC-MS. It was validated in a method validation study on roasted coffee, soluble coffee, coffee substitutes and coffee products with ranges from 53 μg/kg to 612,1 μg/kg.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 3696, Water for analytical laboratory use — Specification and test methods
3 Terms and definitions
No terms and definitions are listed in this document.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— IEC Electropedia: available at http://www.electropedia.org/
— ISO Online browsing platform: available at http://www.iso.org/obp
4 Principle
The coffee sample is extracted with water or, in the case of soluble products, dissolved in water. A clean-up by solid phase extraction is employed to remove interfering matrix compounds. Two alternative methods can be used for the determination: high-performance liquid chromatography with mass spectrometric detection (HPLC-MS/MS) or, after a bromination of the acrylamide, gas chromatography with mass spectrometric detection (GC-MS). In both cases, isotopic labelled internal standard solutions
are used.
5 Reagents
WARNING — In view of health risks when working with acrylamide, appropriate preventive and protection measures shall be taken, such as using a fume cupboard, aspirating acrylamide-containing solutions only with a pipette, and avoiding skin and eye contact or inhalation of acrylamide-containing vapour.
5.4.1 Acrylamide stock solution, mass concentration ρ = 1 000 μg/ml. Weigh (0,10 ± 0,001) g of acrylamide into a 100 ml one-mark volumetric flask and swirl it in 30 ml of water in order to dissolve the acrylamide. Fill up to the mark with water and mix well. The stock solution is stable for at least 3 months when stored protected from light at a maximum of 6 °C. Alternatively, a commercially available solution with a mass concentration of ρ = 1 000 µg/ml may be used. The information of the manufacturer regarding the stability of the solution shall be observed.
5.4.2 Acrylamide calibration solution, ρ = 10 μg/ml. Using a pipette, transfer (1,0 ± 0,001) ml of the acrylamide stock solution (5.4.1) into a 100 ml one-mark volumetric flask and fill up to the mark with water. This solution shall be stored protected from light at a maximum of 6 °C and shall be freshly prepared every working day. Depending on the working range, more dilution steps might be necessary.