BS ISO 21160:2018 pdf download.Cigarettes — Determination of selected carbonyls in the mainstream smoke of cigarettes — Method using high performance liquid chromatography
1 Scope
This document specifies a method for the determination of selected carbonyls (formaldehyde,acetaldehyde, acetone, acrolein, propionaldehyde, crotonaldehyde, 2-butanone and n-butyraldehyde) as their 2,4-dinitrophenylhydrazones in mainstream smoke using reversed phase HPLC-UV/DAD.
This method is applicable to cigarettes with nicotine-free dry particulate matter (NFDPM) yields between 1 mg/cigarette and 15 mg/cigarette using reversed phase HPLC-UV/DAD.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 3308, Routine analytical cigarette-smoking machine — Definitions and standard conditions
ISO 3402, Tobacco and tobacco products — Atmosphere for conditioning and testing
ISO 4387, Cigarettes — Determination of total and nicotine-free dry particulate matter using a routine analytical smoking machine
ISO 8243, Cigarettes — Sampling
3 Terms and definitions
No terms and definitions are listed in this document.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https://www.iso.org/obp
— IEC Electropedia: available at http://www.electropedia.org/
4 Principle
Cigarettes are smoked on a standard smoking machine as specified in ISO 3308 that has been fitted with impingers, but without a glass fibre filter pad as described in ISO 3308 (Cambridge filter pad; CFP, for example of equivalent product) and the filter pad holder, under the ISO 3308 smoking regime. The carbonyls in mainstream tobacco smoke are trapped by passing each puff through an impinger device containing an acidified solution of 2,4-dinitrophenylhydrazine (DNPH) in 1:1 acetonitrile:water. An aliquot of the smoke extract is then syringe-filtered and diluted with 1 % tris-(hydroxymethyl)- aminomethane in aqueous acetonitrile.
7.2.1 DNPH solution (using phosphoric acid)
Add approximately 150 ml deionized water to a 200 ml volumetric flask, then carefully add 28 ml of 85 % phosphoric acid and mix the solution. Make up the solution to volume with deionized water. Weigh approximately 6,8 g (24,0 mmol should be achieved) of DNPH (approximately 30 % water) into a 2 l amber volumetric flask and add 1 l of acetonitrile. Dissolve DNPH by alternately gently swirling the flask. Make sure there are no crystals remaining. WARNING — Do not sonicate as a precipitation of DNPH may occur. If using re-crystallized DNPH, weigh 4,8 g to achieve the same molarity (see Annex A). After the DNPH is dissolved, add 58 ml of the diluted phosphoric acid solution while gently mixing. Dilute to volume with deionized water. The colour of the solution will become bright orange upon addition of the deionized water. The addition of acid or water will cool the solution and may initiate the precipitation of the DNPH. Add the acid or water slowly. Gentle swirling may be required to maintain the solution at room temperature and to prevent the precipitation of DNPH. If crystals appear, do not sonicate. Store the solution in a 4 l amber bottle at room temperature in the dark to prevent or significantly reduce the chances of DNPH precipitation. This solution, if properly sealed, will remain stable for one week.
BS ISO 21160:2018 pdf download
