ISO 19746:2017 pdf download

ISO 19746:2017 pdf download.Determination of urea content in urea-based fertilizers by high performance liquid chromatography (HPLC)
1 Scope
This document specifies the test procedure for determining the urea content in urea-based fertilizers, including urea, urea aldehydes [methylene urea fertilizers, isobutylene diurea (IBDU]), crotonylidene diurea (CDU)], urea triazone fertilizers, urea ammonium nitrate (UAN), sulfur- and polymer-coated urea [SCU and PCU), as well as compound fertilizers containing urea. The method is based on High Performance Liquid Chromatography (HPLC). The proposed method is an extension of the AOAC Official Method 2003.14 which was collaboratively studied for the “Determination of Urea in Water-Soluble Urea-Formaldehyde Fertilizer Products and in Aqueous Urea Solutions” in 2003. The method was published in the Journal of AOAC in 2004[4] and was . granted the First Action in 2003 and the Final Action in 2008. This method also applies to the determination of biuret content in urea containing fertilizer with the results published in the J. AOAC in 2014[5]. This method was adopted by the International Organization for Standardization [ISO) as a Committee Draft [ISO/CD 18643) in 2014, and after review and the Ring Test Analyses[6]. NOTE This HPLC method can also be utilized to analyse Crotonylidene diurea (CDU) and Isobutylidene diurea (IBDU) contents within those above-mentioned fertilizers, in addition to EN 15705[1].
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
– ISO 3696:1995, Water for analytical laboratory use-Specification and test methods
3 Terms and definitions .
No terms and definitions are listed in this document.
ISO and IEC maintain terminological databases for use in standardization at the following addresses: .
– IEC Electropedia: available at http://www.electropedia.org/
– ISO Online browsing platform: available at http://www.iso.org/obp
4 Principles
The urea content in urea-based fertilizer is extracted by aqueous acetonitrile mobile phase and separated from other contents by High Performance liquid chromatography (HPLC) on an aminopropyl column. The urea peak is detected by a UV detector attached to the HPLC.
5 Reagents
WARNING一Acetonitrile is flammable and toxic. The related operations shall be performed in a laboratory fume hood. This document does not point out all possible safety problems, and the user shall bear the responsibility to take proper safety and health measures, and ensure the operations are compliant with the conditions stipulated by the related laws and regulations of the state.