ISO 22718:2015 pdf download

ISO 22718:2015 pdf download.Cosmetics — Microbiology — Detection of Staphylococcus aureus
1 Scope
This International Standard gives general guidelines for the detection and identification of the specified microorganism Staphylococcus aureus in cosmetic products. Microorganisms considered as specified in this International Standard might differ from country to country according to national practices or regulations. In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis to determine the types of cosmetic product to which this International Standard is applicable. Products considered to present a low microbiological (see ISO 29621) risk include those with low water activity, hydro-alcoholic products, extreme pH values, etc. The method described in this International Standard is based on the detection of Staphylococcus aureus in a non-selective liquid medium (enrichment broth), followed by isolation on a selective agar medium. Other methods may be appropriate dependent on the level of detection required. NOTE For the detection of Staphylococcus aureus, subcultures can be performed on non-selective culture media followed by suitable identification steps (e.g. using identification kits). Because of the large variety of cosmetic products within this field of application, this method may not be appropriate for some products in every detail (e.g. certain water immiscible products). Other International Standards (ISO 18415) may be appropriate. Other methods (e.g. automated) may be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable.
11.3.1 Procedure
11.3.1.1 In tubes of 9 ml of diluent, prepare a dilution of the calibrated suspension in order to obtain a final count between 100 CFU per ml and 500 CFU per ml. To count the final concentration of viable microorganisms in the diluted calibrated suspension, transfer 1 ml of the suspension into a Petri dish and pour in 15 ml to 20 ml of the melted agar medium kept in a water bath at no more than 48 °C. Let solidify and then incubate at 32,5 °C ± 2,5 °C for 20 h to 24 h.
11.3.1.2 Prepare in duplicate the initial suspension in the conditions chosen for the test (at least 1 g or 1 ml of product under test, defined volume of enrichment broth) in a tube or flask. When using the membrane filtration method, filter in duplicate at least 1 ml of product under test and transfer each membrane to a tube or flask containing the enrichment broth in the conditions chosen for the test.